Comparitive evaluation of antimicrobial efficacy of 0.1% octenidine dihydrochloride, 2% chlorhexidine and 2% chitosan against E.faecalis within the dentinal tubules

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Original Article

Author Details : Akansha Joon, Ambica Khetarpal*, Sanju Dahiya

Volume : 5, Issue : 4, Year : 2020

Article Page : 192-199

https://doi.org/10.18231/j.ijce.2020.047

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Abstract

Introduction : Root canal treatment is a type of endodontic therapy used to perform for the elimination
of the micro-organisms by standardized mechanical instrumentation and adequate cleaning and shaping of
the infected root canal. The readily available root canal irrigants which are used in endodontic therapy have
shown somewhat toxic and harmful side effects when used at certain concentration, some of the endodontic
irrigants have shown species specific resistance to a particular microbial load that’s why there is a need to
discover newer irrigants which are non toxic, effective and can be used safe to use.
Aim: In vitro evaluation and comparision of the antimicrobial efficacy of 0.1% octenidine dihydrochloride
(OCT), of 2% chlorhexidene (CHX) and 2% chitosan as root canal irrigant against E. faecalis within the
dentinal tubules at 200m and 400mm depth.
Materials and Methods: Seventy two freshly extracted mandibular molars were decoronated and the
mesial root specimen was standardized till working length. E.faecalis (strain MTCC 439) was grown on
brain heart infusion sheep blood agar plate until seven days and the decoronated root specimens collected
were divided into four groups (n=18) based on irrigation protocol: Group 1 – 2% chlorhexidene (CHX),
Group 2 - 0.1% octenidine dihydrochloride (OCT), Group 3- 2% chitosan and Group 4 – normal saline. Each specimen was irrigated with particular irrigant till 3 minutes. Dentin shavings were obtained from
root specimens using diamond disc and these dental chips were used to calculate the colony forming unit
at 200m and 400mm depth. The data obtained after the experiment was statistically analyzed.
Results: Non significant difference has been shown by group 1when compared with group 2 while
significant difference has been shown by group 3 when compared with group 1 and group 2 by taking
into account that group 4 is taken as control group. Significant difference was found when all the groups
1-4 were compared at 200 and 400 mm depth.
Conclusion: It was observed and evaluated that antimicrobial efficacy of 2% chitosan against e. faecalis
was found to be higher as compred to 2% chlorhexidene (CHX) and 0.1% octenidine dihydrochloride
(OCT) both at 200m and 400mm depth.

Keywords: Enterococcus faecalis, Antimicrobial, Octenidene Dihydrochloride, Chlorhexidene, Chitosan.

How to cite : Joon A, Khetarpal A, Dahiya S, Comparitive evaluation of antimicrobial efficacy of 0.1% octenidine dihydrochloride, 2% chlorhexidine and 2% chitosan against E.faecalis within the dentinal tubules. IP Indian J Conserv Endod 2020;5(4):192-199

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